United Therapeutics Corporation
PRE-CONDITIONED EXTRACELLULAR VESICLES AND METHODS OF PRODUCTION

Abstract:

A method for treating inflammatory diseases is provided. The method may include administering to a subject in need thereof a therapeutically effective amount of isolated extracellular vesicles or exosomes obtained from mesenchymal stem cells pre-conditioned with at least TNF-.alpha.. The isolated extracellular vesicles or exosomes may exhibit (a) enhanced expression of flotillin-1, (b) enhanced expression of CD73, or (c) enhanced expression of IDO. A method of isolating extracellular vesicles or exosomes capable of treating an inflammatory disease is also provided, wherein the method may include (a) culturing stem cells in a growth medium, (b) then culturing the stem cells in a starve medium supplemented with at least TNF-.alpha., and (c) separating exosomes or extracellular vesicles from the culture. Cultures suitable for treatment are also provided.