Yield10 Bioscience, Inc.
GENETICALLY ENGINEERED LAND PLANTS THAT EXPRESS LCID/E PROTEIN AND OPTIONALLY A CCP1 MITOCHONDRIAL TRANSPORTER PROTEIN AND/OR PYRUVATE CARBOXYLASE

Abstract:

A genetically engineered land plant that expresses an LCID/E protein is provided. The plant comprises a modified gene for the LCID/E protein. The LCID/E protein comprises (i) LCD of Chlamydomonas reinhardtii of SEQ ID NO: 4, (ii) LCIE of Chlamydomonas reinhardtii of SEQ ID NO: 5, or (iii) an algal or plant ortholog of LCID/E. The LCID/E protein is localized to chloroplasts of the plant based on a plastidial targeting signal. The modified gene for the LCID/E protein comprises (i) a promoter and (ii) a nucleic acid sequence encoding the LCID/E protein. The promoter is non-cognate with respect to the nucleic acid sequence encoding the LCID/E protein. The modified gene for the LCID/E protein is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the LCID/E protein. Optionally, the plant also expresses a CCP1 mitochondrial transporter protein and/or pyruvate carboxylase.