Zymergen Inc.
GENOTYPING EDITED MICROBIAL STRAINS

Abstract:

The present invention relates to methods for genotyping microbial host cells that have been subjected to metabolic engineering. The methods provided herein allow detection of genetic edits in the genome of a microbial host cell using PCR-based genome enrichment following appendage of a common priming site. The compositions and methods of the present invention can be used to confirm engineered metabolic diversity as well as ectopic insertions. Kits for performing the methods are also disclosed.