Zymergen Inc.
MODULAR UNIVERSAL PLASMID DESIGN STRATEGY FOR THE ASSEMBLY AND EDITING OF MULTIPLE DNA CONSTRUCTS FOR MULTIPLE HOSTS

Abstract:

The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro. The disclosure further describes modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations.