Zymergen Inc.
Method for counterselection in microorganisms
Last updated:
Abstract:
The present disclosure is directed to methods of scarless genomic engineering in microorganisms, such as Bacillus, and provides for new molecular tools and methods which enable scarless genetic editing using at least one counterselectable marker that has been codon optimized for the microorganism. The disclosure allows for the high-throughput introduction of stable genetic edits to a genome using either plasmid or linear DNA constructs for genetic engineering.
Status:
Grant
Type:
Utility
Filling date:
9 Aug 2021
Issue date:
12 Apr 2022